400 meter Sen Women, Heat:1 of 3 ; Official result
| Rank | Lane | ID | Name | PB | Team | Cat | Result | Info | Video |
| 1 | 1 | 720 | Justine Palframan | 52.88 | KZN | Jun | 54.92 | Q | |
| 2 | 3 | 514 | Alet Van Wyk | 54.92 | WPA | Sen | 55.03 | Q | |
| 3 | 7 | 718 | Zoe Engler | 55.04 | KZN | Jun | 55.30 | q | |
| 4 | 2 | 335 | Tihanna Vorster | 56.46 | AGN | Sen | 56.21 | ||
| 5 | 5 | 770 | Silba Tjingaete | NAM | Sen | 57.19 | |||
| 6 | 4 | 659 | Loubser Jolanda | 58.97 | EPA | Sen | 58.33 | ||
| 7 | 6 | 423 | Senzeni Mngxanyelwa | 1:13.33 | TRA | Sen | 1:13.18 |